Chemistry Glossary: Search results for 'reverse reaction'

How can synthesis and decomposition reactions be the reverse of each other?
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Synthesis and studies on forward and reverse reactions …

Some chemical reactions are reversible, and when the rate of the forward reaction equals the rate of the reverse reaction, the process has reached equilibrium.

Secondly, the reverse reaction involving release of heat will not occur until the products react to form reactants again releasing the binding energy.
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When you do a synthesis reaction, ..

PrimeScript Reverse Transcriptase has exceptionally strong strand displacement activity and enables efficient preparation of cDNA up to 12 kb in length. It is robust, versatile and well-suited for applications requiring full-length cDNA such as preparation of cDNA libraries and other techniques involving first strand cDNA synthesis (RT-PCR, preparation of cDNA probes, real-time quantitative RT-PCR). PrimeScript RTase can be used for performing a reverse transcription reaction with any RNA template including GC-rich templates and RNAs with high levels of secondary structure. This enzyme is a modified, recombinant MMLV (Moloney Murine Leukemia Virus) reverse transcriptase and is verified to be RNase H Minus. Because of the excellent extension capability of PrimeScript Reverse Transcriptase, preparation of cDNA can be performed at a lower temperature (42°C), decreasing the risk of RNA degradation that can occur during conventional reactions performed at higher temperatures.

08/03/2017 · Understand the definition of reversible reaction in ..
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The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). Reverse transcriptases (RTs) use an RNA template and a short primer complementary to the 3' end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. Alternatively, the first-strand cDNA can be made double-stranded using DNA Polymerase I and DNA Ligase. These reaction products can be used for direct cloning without amplification. In this case, RNase H activity, from either the RT or supplied exogenously, is required.

Reverse transcription and polymerase chain reaction: principles and applications in dentistry
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