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Phytohormones and their metabolic engineering for …

WHO | WHO definitions of genetics and genomics

Increasing photosynthetic efficiency has not yet been addressed by conventional breeding methods, though it has the potential to increase yields and reduce the use of water and nitrogen. Team members will apply recent advances in photosynthesis research and crop bioengineering to the RIPE project. In addition, computer simulation models of the highly complex photosynthetic system, combined with practical engineering, will identify the best targets for improving photosynthesis efficiency.

Biotechnology for the Development of Drought Tolerant Crops

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Increasing photosynthetic efficiency has not yet been addressed by conventional breeding methods, though it has the potential to increase yields and also increase the efficiency with which crops use water and nitrogen. Team members will apply recent advances in photosynthesis research and crop bioengineering to the RIPE project. In addition, computer simulation models of the highly complex photosynthetic system, combined with practical engineering, will identify the best targets for improving photosynthesis efficiency.

Improving Photosynthesis and Crop Yield | …

In photosynthetic organisms, D-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the major enzyme assimilating atmospheric CO2 into the biosphere. Due to the wasteful oxygenase activity and slow turnover of Rubisco, the enzyme is among the most important targets for improving the photosynthetic efficiency of vascular plants,. It is also anticipated that introducing the CO2-concentrating mechanism (CCM) from cyanobacteria into plants could enhance crop yield-. However, the complex nature of Rubisco’s assembly has made manipulation of the enzyme extremely challenging, and attempts to replace it in plants with the enzymes from cyanobacteria and red algae have not been successful,. Here we report two transplastomic tobacco lines with functional Rubisco from the cyanobacterium Synechococcus elongatus PCC7942 (Se7942). We knocked out the native tobacco gene encoding the large subunit of Rubisco by inserting the large and small subunit genes of the Se7942 enzyme, in combination with either the corresponding Se7942 assembly chaperone, RbcX, or an internal carboxysomal protein, CcmM35, which incorporates 3 small subunit-like domains,. Se7942 Rubisco and CcmM35 formed macromolecular complexes within the chloroplast stroma, mirroring an early step in the biogenesis of cyanobacterial β-carboxysomes,. Both transformed lines were photosynthetically competent, supporting autotrophic growth, and their respective forms of Rubisco had higher rates of CO2 fixation per unit of enzyme than the tobacco control. These transplastomic tobacco lines represent an important step towards improved photosynthesis in plants and will be valuable hosts for future addition of the remaining components of the cyanobacterial CCM, such as inorganic carbon transporters and the β-carboxysome shell proteins-.

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